Our new Obvious+ tissue clearing procedure is the only real method that delipidates samples with no modify in morphology and with nominal influence on structural integrity.
Megatome is often a vibrating microtome built to portion a broad selection of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With large blade vibrating frequency and minimized blade deflection, Megatome allows substantial-throughput tissue sectioning with uniform floor profile, together with negligible tissue damage and information loss.
Antibodies might consider weeks to diffuse via only a few millimeters of tissue, by using a steep labeling gradient from floor to Main.
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SE works by using a rotational electric subject to disperse remarkably electromobile molecules (for instance antibodies or surfactant micelles) through a porous sample without having harmful electrically charged buildings throughout the tissue. This permits 2-four working day clearing of intact organs,
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Megatome is actually a novel microtome that allows for prime-precision sectioning of a wide range of tissue samples – from organoids, to arrays of animal organs, to intact human Mind hemispheres – with nominal tissue damage and knowledge reduction.
eFLASH is really a rapid tissue labeling strategy that allows for uniform total-organ staining in 20 rounds of labeling.
Totally delipidate total mouse brains or comparably sized samples in just one working day with SmartBatch+, or in one 7 days with our passive clearing kit.
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SHIELD avoids the variability of hydrogel embedding and the information loss from PFA preservation, defending specimens for many rounds of processing.
Leverage the Distinct+ tissue clearing approach, in conjunction with eFLASH and patented stochastic electrotransport systems, to rapidly very clear and label complete organs. Critical highlights and options consist of:
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